homebotanik AG Van Bel
Text and images are subject to copyright by the
American Society of Plant Physiologists and are reprinted with
permission. The original text and photographs have appeared in
The Plant Cell 10, 35-50 (1998) under the title: "Sieve tubes in action"
by Michael Knoblauch and Aart.J.E. van Bel, Institut für Allgemeine
Botanik, Senckenbergstrasse 17, 35390 Giessen, Germany. Additional
time scans give information about the laser-induced structural changes in
the sieve elements.
Figure 1 : CLSM images of sieve elements in tissue slices
Figure 2 : Reconstruction of the Structure of sieve elements exposed to different degrees and forms of injury.
Figure 3 : In situ observation of functional sieve elements in intact plants.
Figure 4 : CLSM-images of living actually translocating sieve elements
Figure 5 : Double stains of living actually translocating sieve elements.
Figure 6 : Response of actually translocating sieve elements to intense laser light
Figure 7 : Reaction of intact sieve elements to injury incurred by a "medium tip" microelectrode.
Figure 8 : Reaction of intact sieve elements to injury incurred by a "small tip" microelectrode.
Figure 9 : Reaction of intact sieve elements to injury incurred by a "coarse tip" microelectrode.
Figure 10 : Reconstruction of the in vivo sieve element structure in Vicia faba.
Additional time scans (movies)
This film shows a time scan of the reaction of sieve elements to intense laser light (see for further specification of the laser irradiation and a precise description of the events, legend of figure 6 and in Knoblauch and Van Bel, The Plant Cell 10, 35-50, 1998). The reaction probably results from the heat produced by the laser beam in confocal laser scanning microscopy.
short movie 275 KB
long movie 1.5 MB
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